Strain Information | |
---|---|
Image | |
BRC No. | RBRC09946 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6D2-Cmtm2a<em1Osb> Cmtm2b<em1Osb> |
Former Common name | Cmtm2a<-15/wt> Cmtm2b<-8/wt> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2013. Fertilized eggs derived from BDF1×BDF1 were used. Mixed genetic background. |
Strain description | Cmtm2a/Cmtm2b mutant mice generated by the CRISPR/Cas9 technique. 15 bp and 8 bp deletion at Cmtm2a and Cmtm2b, respectively.Cmtm2a<em1Osb>: 15 bp deletion at exon 1 of Cmtm2a.ctgtctggttcctgttgcatggaggagatcctgctggtccactagatgaggtgggctcgcgccaatcaagagcaagcgttagaattctgcagttccAGTGGTCAGTTGAGAGCCACCCTTGAACAGGTCAGGAGACACCAGGCTCTCTCTCGAGCTGGGCCTGTTCAGCGACTGCAGACAAGGCTGGGCGGTAGGAAGCTGAGAACTACCTGCTAACAACCATGGCAGCACCGATAAAGTTTCCATTTCGGCCCAGAGGAGGTCAGCCCAGAGAGGACACGACCCCAAAAAGGGGGCTTCGACGCTATCTATTGGAATTGAAAGAGAGCAATAAAGAGTTCTGGCTGTCAGGACACGCCGTGTTCAAGCTTCTGTCTTTGgtatactggagtggagtcgatatgttgttgggattggagaggtcctcagaagtaggggagagtgcttgcagcagctcaggtgtatctatttttccacccagGGCTGCATGATCTCTGCACTAGACTACTTTGAAACAATGCTCCCACATCCGGTCCTGATCCTGCTCATCTGTATGGAAGCGGCAATCTGCATATTCTTCATCTTCCTTAACACCTTAGCCATCAACAGATACATTCCCTTCGTCTTCTGGCCTATGGCGgtaagtaaagactgtgggggaggggggtaatgtgggtcacattctaacaactggaaaaaagacaacctcacctcctctaccggtgctcagggctgggaagctgggttggagaggaaaagccaggcaggagatgctcccaaacatgtaactgtgctaggtcaaagaaagaagacgcaaaggctttgctgtcctcgCmtm2b<em1Osb>: 1 bp deletion at exon 1 of Cmtm2b.gcatggaggagatcctgctggtccactagatgaggtgggctcgcgccaatcaagagcaagcgttagaattccgcagtCCCAGTGGTCAGTTGAGAGCCACCCTTGAACAGGTCAGGAGACACCAGGCTCTCTCTCGAGCTGGGCCTGTTCAGCGACTGCAGACAAGGCTGGGCGGTTGGAAGCTGAGAACTACCTGCTAACAACCATGGCAGCGCCCGCGCCGCGAGCCCGGACAGGTGGCAAGAAGAAAGATGAAAGAAGAGGGTTCAAAGGCTACAAGTGGGAATTCAGAGACAGCAATAAAGACTTCTGGGCGCAGGGACATGCAGAGTGCAAGTCTCTGATTATGgtaaactagattggaatgacagaggtgttggtgcgggtgggggtggggttcctcaaaagcagggtagagcccttgcaacccctccggtgtacgtatatttttccattcagATCCTCCTCATTGCTGCAATGGTCTGTTTCCAAAGAGTGGCCACTCATCCAATTGTAATCCTGCTGCTTACTATGGAACTGTCAATCTGTGCTTTCTTCTTCTTCCTCTACTCCTTAGCCATCAACAGATACATTCCCTTCGTCTTCTGGCCTATGATGgtaagtaaaaaacggtaggaaggtgatgtgggtcacattctagccactggaggaaaaaaaaaaaaagacaaccttgccccctctaccggtgctcgggctgggaagctgggttggagagggaaagccaggcaggagatgctcccaaaaaatgtaactgtgctgggtcaaagagagaagaaccaaaggctttgctgtcc |
Colony maintenance | |
References | J. Cell Sci., 131(19) (2018). pii: jcs221481. doi: 10.1242/jcs.221481. 30209135 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cmtm2aCKLF-like MARVEL transmembrane domain containing 2A8Cmtm2a<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cmtm2bCKLF-like MARVEL transmembrane domain containing 2B8Cmtm2b<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
---|---|
Donor DNA | human hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of Cmtm2a and Cmtm2b gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Cell Sci. 2018 Oct 8;131(19). pii: jcs221481. doi: 10.1242/jcs.221481.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
|
BRC mice in Publications |
---|
No Data |