Strain Data Sheet

RBRC09959

Strain Information

Image
BRC No.RBRC09959
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameSTOCK Ace<em1Osb>
Former Common nametACE<-37/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were backcrossed to BDF1 for several generations. Mixed genetic background.
Strain descriptionAce mutant mice generated by the CRISPR/Cas9 technique. 37 bp deletion at exon 1.Ace<em1Osb>: 37 bp deletion at exon 1.gggtatgtctttctctcagggtcctagagacagtgtgcccaagcctgagggcccttggggtcaggctggctggcacattgctctatgaggtcacactgcaggcttggctcttattggccggtgatgggagcttcagggctctgctttcctgcggccATGGGCCAAGGTTGGGCTACTCCAGGACTGCCCAGCTTCCTCTTTCTGCTGCTCTGCTGTGGGCACCATCTGCTGGTCCTGAGTCAGGTGGCCACTGACCACGTGACAGCCAACCAGGGGATAACAAACCAGGCAACAACCAGAAGCCAGACAACAACTCACCAAGCAACCATTGACCAGACAACCCAGATCCCAAgtgggactcttggaagggggcagggccatgagggggcaaggagccaggggccagcaggagggaactccaatgagaccacaccctgtggtaaggaaggcaaggcctgtctgttttcctcctcacctcctgcagacctagagactgatgaagccaaggc
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 116(37):18498-18506 (2019). doi:10.1073/pnas.1908736116. 31455729

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aceangiotensin I converting enzyme (peptidyl-dipeptidase A) 111Ace<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of Ace gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18498-18506. doi: 10.1073/pnas.1908736116.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data