Strain Data Sheet

RBRC09990

Strain Information

Image
BRC No.RBRC09990
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6D2;B6-Pate4<tm1a(KOMP)Osb>
Former Common namePate4
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2012. EGR-G101 ES cells derived from a Tg mouse in C57BL/6N background were used. Mice were crossed to B6D2F1. Mixed genetic background.
Strain descriptionPate4 knockout mice.
Colony maintenance
ReferencesBiol. Reprod., 100(4):1035-1045 (2019). 30452524

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pate4prostate and testis expressed 49Pate4<tm1a(KOMP)Osb>targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNAchicken beta-actin promoter, rabbit beta-globin polyadenylation signal, CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter, mouse Pate4 genomic DNA, mouse En2 splice acceptor (En2 intron2/exon 3 splice acceptor), EMCV Internal ribosomal entry site (IRES), E.coli. lacZ, SV40 polyadenylation site, human Beta-actin promoter, E.coli. Neomysin phosphotransferase gene (neoR), Yeast FRT site, Phage P1 loxP site
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Reprod. 2019 Apr 19;100(4):1035-1045Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data