Strain Data Sheet

RBRC10004

Strain Information

Image
BRC No.RBRC10004
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;Cg-Hsf4<tm1Anak>
Former Common nameHSF4KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Mitsuaki Fujimoto and Akira Nakai, Yamaguchi University in 2004. TT2 ES cells(C57BL/6 x CBA F1 origin)were used. Mice were backcrossed to ICR.
Strain descriptionHsf4 (heat shock transcription factor 4) knockout mice. Homozygous mutants display caratacts.
Colony maintenance
ReferencesEMBO J., 23, 4297-4306 (2004). 15483628

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hsf4heat shock transcription factor 48Hsf4<tm1Anak>targeted mutation 1, Akira Nakai

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase I (PGK) promoter, E. coli neomycin resistance gene, mouse phosphoglycerate kinase I (PGK) polyA signal, mouse Hsf4 genomic DNA
Research applicationDevelopmental Biology Research
Mouse Models for Human Disease
Sensorineural Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 23, 4297-4306 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAkira Nakai (Yamaguchi University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data