Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC10015  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Bcl6<tm1.1Toka>  
Former Common name YFP-Bc16  
H-2 Haplotype No Data  
ES cell line Bruce 4 [B6.Cg-Thy1]  
Background strain C57BL/6JJcl  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain description The monomeric yellow fluorescent protein (YFP) gene was inserted in-frame right after the initiation codon of the Bcl6 gene such that the product of this allele would be the YFP-Bcl6 fusion protein. Bcl6 protein expression can be analyzed by flow cytometry. Fluorescence of YFP-Bcl6 in most most cell types is too dim to be analyzed by microscopy unless cells/tissues are stained with anti-GFP and a fluorescent dye-labeled secondary antibody.Homozygous animals.
YFP-Bcl6 is functionally compromised, possibly because fusion with YFP near the BTB domain of Bcl6 might impair recruitment of the corepressor molecules or disrupt the formation of Bcl6 homodimers or heterodimers with other BTB domain-containing transcription factors. Therefore, homozygous animals are hypomorphic. Heterozygous animals are largely normal.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Bcl6  
Symbol name B cell leukemia/lymphoma 6  
Chromosome 16  
Common name No Data  
Symbol description No Data  
 2 Symbol EYFP  
Symbol name Enhanced Yellow Fluorescent Protein (Jellyfish)  
Chromosome 16  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 16  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Immunity, 34(6), 961-972 (2011).
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Mouse of the Month Jan 2019  
 3 Genotyping protocol <PCR>  
Depositor RIKEN   
Strain Status /
Availability
(Expected delivery)

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