Strain Information | |
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Image | |
BRC No. | RBRC10065 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6;B6D2-Tg(CAG-Hist1h3f)17Ibbr |
Former Common name | Flag-H3.2 transgenic mouse |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Fugaku Aoki, Machika Kawamura and Tomohiko Akiyama, The University of Tokyo in 2013. Fertilized eggs derived from C57BL/6 (male) X B6D2F1 (female) were used. Backcrossed to C57BL/6J for 3 generations. Mixed genetic background. |
Strain description | Transgenic mice expressing mouse histone H3.2(Hist1h3f histone cluster 1, H3f)under the control of the CAG promoter. Flag tag is fused to histone H3.2 N-terminally. |
Colony maintenance | Carrier x Noncarrier [or Crossing to C57BL/6JJmsSlc] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)UNFLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hist1h3fhistone cluster 1, H3fUNHist1h3ftransgene insertion 17, Fugaku AokiCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Flag-tag cDNA, Mouse Histone H3.2 cDNA (Hist1h3f) |
Research application | |
Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
Depositor | Fugaku Aoki (The University of Tokyo) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |