Strain Data Sheet

RBRC10129

Strain Information

Image
BRC No.RBRC10129
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameSTOCK Gykl1<em1Osb>
Former Common nameGykl1<-4/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Keisuke Shimada and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2017. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background.
Strain descriptionMutant harboring 4 bp deletion at the Gykl1 gene generated by the genome editing technique. Gykl1<em1Osb>; 4 bp deletion GCAAGACCCAAAGGCAATCCTGCAGTCTGTGTACGAGTGTATAGAGAAAGCATGTGAGAAACTCGGACAGCAGAGCATTGATATTTCCAACATAAAAGCTATCGGTGTCACCAACCAGCGGGAAACTACGATAGTCTGGGACAAGTTCACTGGAGAACCTCTCTACAATGCTGTGGTGTGGCTTGACCTAAGGACCCAGTCTACTGTGGAGAATCTCAGCAAAAGTATTTCAGTAAGCAATAACTTTGTCAAGAACAAGACAGGCCTTCCAATCAGCACTTATTTTAGTGCCGTGAAACTTCACTGGCTCATTGAAAATGTGAGAAAAGTGCAAAAGGCTATTGAAGATGGCAGAGCTATTTTTGGGACGGTTGATTCGTGGCTTATCTGGTGTATGACTGGAGGTATCAACGGAGGTGTCCACTGTACAGATGTAAGTAATGCCAGCAGGACCATGCTTTTCAACATTCACTCTTTGCAATGGGATGAAGAGCTCTGCGACTTTTTTGGAATTCCAATGACCATCCTTCCCCGAATCC
Colony maintenance
ReferencesJ. Reprod. Dev., 65(2):155-162 (2019). 30662012

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gykl1glycerol kinase-like 118Gykl1<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Mouse a part of Gykl1 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Reprod. Dev., 65(2):155-162 (2019).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data