Strain Information | |
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Image | |
BRC No. | RBRC10136 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;Cg-Col1a1<tm2(tetO-Dek)Hirt> |
Former Common name | iDek |
H-2 Haplotype | |
ES Cell line | KH2[F1:C57BL/6 x 129Sv] |
Background strain | |
Appearance | |
Strain development | Developed by Hiroyuki Tomita, Gifu University in 2010. KH2 ES cell (F1:C57BL/6 x 129/SV) was used. Mice were backcrossed to C57BL/6J for 3 or 4 generations. |
Strain description | A mouse strain in which cDNA coding Dek [DEK oncogene (DNA binding)] with FLAG tag is driven by the TetO promoter. DNA construct was knocked in at the Col1a1 (collagen, type I, alpha 1) locus. |
Colony maintenance | |
References | Cancer Med. 2017 Aug 23. doi: 10.1002/cam4.1157. 28834425 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter DekDEK oncogene (DNA binding)11DekE. coli tetO (tetracycline operator) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)11FLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hyghygromycin phasphotransferase (E. coli)11Hygmouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SAAdenovirus Major late transcript splice acceptor sequence11SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA11 |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) sites, Adenovirus Major late transcript splice acceptor sequence (SA), Simian Virus 40 SV40 polyA, E. coli tetO (tetracycline operator), Mouse Dek cDNA, Synthetic FLAG tag, vector, Escherichia coli ampicillin resistant gene, mouse PGK promoter, E. coli hygromycin resistance gene, mouse Col1a1 genomic DNA |
Research application | FLP/frt system Tet system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cancer Med. 2017 Aug 23. doi: 10.1002/cam4.1157. |
Depositor | Hiroyuki Tomita (Gifu University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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