Strain Information | |
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Image | |
BRC No. | RBRC10148 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Nfe2l1<tm1.2Mym> |
Former Common name | Nrf1-floxed mouse |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Masayuki Yamamoto, Tohoku University School of Medicine in 2007. E14 ES cell line derived from 129P2/OlaHsd was used. Mice were backcrossed to C57BL/6J for 10 generations. Y chromosome is derived from C57BL/6J. |
Strain description | Floxed Nrf1 (Nfe2l1, nuclear factor, erythroid derived 2,-like 1) mouse. Exon 4 was flaked by two loxP sites. A Neo gene cassette was removed by crossing with a Ayu-Cre line. Konckout mice at whole body are embryonically lethal. |
Colony maintenance | |
References | J. Biol. Chem., 283, 33554-33562 (2008). doi: 10.1074/jbc.M804597200. 18826952 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Nfe2l1nuclear factor, erythroid derived 2,-like 111Nfe2l1<tm1.2Mym>targeted mutation 1.2, Masayuki Yamamoto Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)11EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)11 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP |
Ordering Information | |
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Donor DNA | Bacteriophage P1 loxP sites, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), A. coerulescens EGFP cDNA, mouse Nfe2l1 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 283, 33554-33562 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For two years after deposition, the RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Masayuki Yamamoto (Tohoku University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |