Strain Data Sheet

RBRC10148

Strain Information

Image
BRC No.RBRC10148
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Nfe2l1<tm1.2Mym>
Former Common nameNrf1-floxed mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Masayuki Yamamoto, Tohoku University School of Medicine in 2007. E14 ES cell line derived from 129P2/OlaHsd was used. Mice were backcrossed to C57BL/6J for 10 generations. Y chromosome is derived from C57BL/6J.
Strain descriptionFloxed Nrf1 (Nfe2l1, nuclear factor, erythroid derived 2,-like 1) mouse. Exon 4 was flaked by two loxP sites. A Neo gene cassette was removed by crossing with a Ayu-Cre line. Konckout mice at whole body are embryonically lethal.
Colony maintenance
ReferencesJ. Biol. Chem., 283, 33554-33562 (2008). doi: 10.1074/jbc.M804597200. 18826952

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nfe2l1nuclear factor, erythroid derived 2,-like 111Nfe2l1<tm1.2Mym>targeted mutation 1.2, Masayuki Yamamoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)11EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Ordering Information

Donor DNABacteriophage P1 loxP sites, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), A. coerulescens EGFP cDNA, mouse Nfe2l1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 283, 33554-33562 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For two years after deposition, the RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasayuki Yamamoto (Tohoku University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data