Strain Data Sheet

RBRC10171

Strain Information

Image
BRC No.RBRC10171
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gt(ROSA)26Sor<tm3(rtTA3)Yasu> Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>
Former Common nameRosa26-LSL-rtTA3/ Col-tetO-OSKM
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeveloped by Hirofumi Shibata and his colleagues in 2015, and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1 were used. Mixed genetic background.
Strain descriptionCompound mouse line harboring the following two loci; 1, loxP-Pgk-neo-loxP-rtTA3 is knocked in at the Rosa26 locus, 2, tetO-OSKM-ires-mCherry is knocked in at the Col1a1 locus. OSKM stands for Oct3/4, Sox2, Klf4 and c-Myc.
Colony maintenance
ReferencesNature Commun., 9, 2081 (2018). 29802314

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Col1a1collagen, type I, alpha 111Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>targeted mutation 4, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm3(rtTA3)Yasu>targeted mutation 3, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
E2AEquine rhinitis A virus (ERAV) 2A peptideE2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2Rabbit globin polyAHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hyghygromycin phasphotransferase (E. coli)Hyg

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptideP2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor (adenovirus)SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)T2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)mCherry

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (Klebsiella pneumoniae)mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 pA

Ordering Information

Donor DNAadenovirus type2 Splicing acceptor (SA) [Rosa26, Col1a1 locus], P1 Bacteriophage loxP sites, mouse Pgk1 promoter, Klebsiella pneumoniae (Bacterial transposon Tn5) Neomycin registance gene (Neo), SV40 pA, Escherichia coli K-12 strain derivative rtTA3, Escherichia coli K-12 strain derivative Hygromycin registance gene, CMV promoter, E. coli tetO (tetracycline operator), yeast Frt site, Escherichia coli ampicillin registance gene, mouse Oct3/4, mouse Sox2, mouse Klf4, mouse c-Myc, Porcineteschovirus-1 P2A,Thoseaasignavirus T2A,Equine rhinitis A virus E2A, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Discosoma sp. mCherry cDNA, Rabbit globin polyA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Commun., 9, 2081 (2018).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasuhiro Yamada (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data