Strain Information | |
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Image | |
BRC No. | RBRC10188 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Gt(ROSA)26Sor<tm1(CAG-3XFLAG/dCas9,-EGFP)Hfuj> |
Former Common name | 3×FLAG-dCas9/CTV: neo (+), GFP (+) |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Hodaka Fujii/Osaka University and Ichiro Taniuchi/RIKEN in 2016. B6JN/1 ES cells [(C57BL/6JJcl x C57BL/6NJcl)F1] were used. C57BL/6 background. This line has been maintained by crossing between heterozygotes and C57BL/6. No obvious phenotype was observed. |
Strain description | Knockin mice for Cre-dependent expression of dCas9 and EGFP. DNA fragment of CAG-loxP-Neo-Stop-loxP-3xFLAG-dCas9-Frt-IRES-EGFP-Frt-pA was knocked in at the Rosa26 locus. |
Colony maintenance | |
References | Genes Cells. 23(4):318-325 (2018). 29480524 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-3XFLAG/dCas9,-EGFP)Hfuj>targeted mutation 1, Hodaka Fujii Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAG3xFLAG tag (synthetic)6FLAGCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter dCas9Streptococcus pyogenes Cas9 mutant6dCas9 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene6herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA6 |
Ordering Information | |
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Donor DNA | adenoviral splice acceptor (SA), CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli neomycin resistance gene, tk polyA, yeast His3 gene, Synthetic 3xFLAG tag, Streptococcus pyogenes Cas9 mutant (dCas9), yeast FRT (flipase recombination target) sites, ECMV internal ribosomal entry site (ires), Aequorea victoria green fluorescent protein (GFP) cDNA, bovine GH polyA, mouse Rosa26 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 23(4):318-325 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Hodaka Fujii (Hirosaki University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Oct 2019 |
BRC mice in Publications |
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No Data |