Strain Information | |
---|---|
Image | |
BRC No. | RBRC10241 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6-Tg(Scn1a-GFP)1Kzy |
Former Common name | |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Ikuo Ogiwara and Kazuhiro Yamakawa, RIKEN BSI in 2008. In the BAC clone (RP23-232A20), GFP was placed at the translation initiation codon at the Exon 1 of the Scn1a gene. Then the BAC DNA construct was injected to the fertilized eggs of C57BL/6J. C57BL/6 genetic background. |
Strain description | Transgenic mice expressing GFP underthe control of the Scn1a (sodium channel, voltage-gated, type I, alpha) gene promoter. Both males and females are fertile. |
Colony maintenance | |
References | Elife, 12:e87495 (2023). 37219072 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse Scn1a promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyAUN |
Ordering Information | |
---|---|
Donor DNA | mouse Scn1a genomic DNA, jellyfish GFP cDNA, SV40 polyA, Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-232A20 |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Elife, 12:e87495 (2023).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Kazuhiro Yamakawa (RIKEN) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |