Strain Data Sheet

RBRC10249

Strain Information

Image
BRC No.RBRC10249
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Stx1b<tm1Tfuj>/211
Former Common nameSyntaxin1B
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Tomonori Fujiwara, Kyorin University in 2013. TT2 ES cells were used. Mice were backcrossed to C57BL/6J for 9 generations. This strain was derived from ES clone #211.
Strain descriptionStx1b (syntaxin 1B) knockout mice. Homozygous mutants die by postnatal day 14. Heterozygous mutnats are viable and fertile, but prone to develop convulsive seizure.
Colony maintenance
ReferencesJ. Neurochem., 30(4) 514-525 (2014). 24666284
J. Neurochem., 156(5) 604-613 (2021). 32858780

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Stx1bsyntaxin 1B7Stx1b<tm1Tfuj>targeted mutation 1, Takefumi Kofuji

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)7GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (MC1 promoter), E. coli neo, Bovine growth hormone polyA, mouse HPC-1/syntaxin1A genome DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurochem., 30(4) 514-525 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTomonori Fujiwara (Kyorin University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data