Strain Data Sheet

RBRC10264

Strain Information

Image
BRC No.RBRC10264
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Ppm1b<tm1.2Tamu>
Former Common namePpm1b2lox/wt mouse, PP2Cβ2lox/wt mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Motoko Ohnishi, Chubu University. Developed by Shinri Tamura, Tohoku University, in collaboration with Jun-ichi Miyazaki, Osaka University. ES cells derived from 129SV were used and then the mice were backcrossed to C57BL/6N over 37 generations. Neo gene fragment was deleted at ES cells.
Strain descriptionPpm1b (protein phosphatase 1B, magnesium dependent, beta isoform) mutant mice. A pair of loxP flanks Exon 4. Homozygous mutants are lethal at around embryonic day 3.5. No expression of Ppm1b was detected from this allele.
Colony maintenance
ReferencesMech. Dev., 124(6), 489-499 (2007). 17499977

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ppm1bprotein phosphatase 1B, magnesium dependent, beta isoform17Ppm1b<tm1.2Tamu>targeted mutation 1.2, Shinri Tamura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence17lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Ordering Information

Donor DNAphage P1 loxP site, phage P1 lox71 site, mouse Ppm1b genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mech Dev., Jul,124(6), 489-499, (2007). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorMotoko Ohnishi (Chubu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data