Strain Information | |
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Image | |
BRC No. | RBRC10266 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Ppm1l<tm1Tkoba>/79 |
Former Common name | Ppm1llox/wt mouse, PP2Cεlox/wt mouse |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Motoko Ohnishi, Chubu University. Developed by Shinri Tamura, Tohoku University, in collaboration with RIKEN CDB in 2006-2007 (CDB0428K). This strain was backcrossed to C57BL/6N over 28 generations. |
Strain description | Ppm1l [protein phosphatase 1 (formerly 2C)-like] knockout mice. The b-galactosidase(LacZ) and neomycin gene cassette were inserted into the 50 UTR sequence of exon 1. Homozygous mutants were born less than the mendelian ratio and died by 4 weeks old. ES clone #79 was used to generate this line. |
Colony maintenance | |
References | FEBS Lett., 590(20), 3606-3615 (2016). 27680501 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ppm1lprotein phosphatase 1 (formerly 2C)-like3Ppm1l<tm1Tkoba>targeted mutation 1, Takayasu Kobayashi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox71mutant lox sequence3lox71 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | phage P1 lox71 site, E. coli lacZ, rabbit beta-globin 3’end, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neo, phage P1 loxP site, PGK3’end, mouse Ppm1l genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Lett., Oct, 590(20), 3606-3615 (2016). |
Depositor | Motoko Ohnishi (Chubu University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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