Strain Information | |
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Image | |
BRC No. | RBRC10268 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Mlc1<tm1.1kftnk> |
Former Common name | Mlc1 tetO knockin |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed and deposited by Kazuhiro Ikenaka, National Institute of Physiological Sciences, and Kenji Tanaka, Keio University. ES cells derived from 129S6/B6 hybrid were used. Mixed genetic background. |
Strain description | A mutant mouse in which the tetracycline operator (tetO) was inserted upstream of the translation initiation site. This strain was generated by crossing RBRC10267 with a Flpe line. |
Colony maintenance | |
References | Glia, 65(1), 150-168 (2017). doi: 10.1002/glia.23084. Epub 2016 Oct 17. 27748972 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Mlc1megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human)11Mlc1<tm1.1kftnk>targeted mutation 1.1, Kenji Tanaka Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tetOtetracycline operator (E. coli)11tetOCMV promoter |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, yeast FRT (flippase recombination target) site, E. coli Tetracycline operator site, CMV minimal promoter, mouse Mlc1 genomic DNA |
Research application | Cre/loxP system FLP/frt system Tet system |
Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
Depositor | Kazuhiro Ikenaka (National Institute for Physiological Sciences) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |