Strain Data Sheet

RBRC10280

Strain Information

Image
BRC No.RBRC10280
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Ahcyl1<tm1Kmik>/1
Former Common nameIRBIT flox mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2009. TT2 ES cells were used and this subline #1 was backcrossed to C57BL/6 for 13 generations. C57BL/6 congenic line.
Strain descriptionhcyl1 (S-adenosylhomocysteine hydrolase-like 1) floxed mice. FRT-flanked Neo gene is placed at intron 1 of the Ahcyl1 gene. A pair of loxP sites flank the FRT-flanked Neo and exon 2 of the Ahcyl1 gene. TT2 ES cells were used and this subline #1 was backcrossed to C57BL/6 for 13 generations. C57BL/6 congenic line.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 103(25):9542-9547 (2006). 16769890
Mol Cell. 22(6):795-806 (2006). 16793548
Proc. Natl. Acad. Sci. USA, 112(17):5515-5520 (2015). 25922519

Health Report

Examination Date / Room / Rack2022/07/11Room:3-BRack:G
2022/04/11Room:3-BRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ahcyl1S-adenosylhomocysteine hydrolase-like 13Ahcyl1<tm1Kmik>targeted mutation 1, Katsuhiko Mikoshiba

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene3mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAP1 phage loxP sites, Saccharomyces cerevisiae frt sites, mouse PGK promoter, Escherichia coli neomycin-resistant gene (Pro/Neo/3'end), mouse PGK pA, mouse Ahcyl1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKatsuhiko Mikoshiba (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data