Strain Information | |
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Image | |
BRC No. | RBRC10289 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129S4-Itpr2<tm1Kmik> |
Former Common name | IP3R2 knockout mice |
H-2 Haplotype | |
ES Cell line | J1 [129S4/SvJae] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2005. J1 ES cells derived from 129S4/SvJae were used. C57BL/6J congenic line by crossing with C57BL/6 for 10 generations. |
Strain description | Itpr2 (inositol 1,4,5-trisphosphate receptor 2) KO mouse. A nls-LacZ gene fragment was inserted downstream of the translation initiation codon. Homozygous mutant exhibit decreased sweating and disturbed calcium signaling in sweat glands. |
Colony maintenance | |
References | Sci. Rep., 11, 8150 (2021). 33854148 Science, 309(5744) 2232-2234 (2005). 16195467 J. Clin. Invest., 124(11) 4773-4780 (2014). 25329695 |
Health Report | |
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Examination Date / Room / Rack | 2024/01/22Room:3-CRack:F |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene6mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)6nls |
Ordering Information | |
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Donor DNA | Mouse PGK promoter, Escherichia coli neomycin-resistant gene, mouse PGK pA, SV40 large tumor nuclear location signal, Escherichia coli lacZ gene, mouse Itpr2 genomic DNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Katsuhiko Mikoshiba (RIKEN) |
Strain Status | Live mice |
Strain Availability | Live mouse (more than 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |