Strain Data Sheet

RBRC10291

Strain Information

Image
BRC No.RBRC10291
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Itpr2<tm1Kmik> Itpr3<tm1Kmik>
Former Common nameIP3R2 IP3R3 double knockout mice
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2005. J1 ES cells derived from 129S4/SvJae were used for Itpr2. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1 were used for Itpr3. C57BL/6J congenic line by crossing with C57BL/6 for 10 generations.
Strain descriptionDouble KO line for Itpr2 (inositol 1,4,5-trisphosphate receptor 2) and Itpr3 (inositol 1,4,5-triphosphate receptor 3). Each modified allele is derived from RBRC10289 and RBRC10290.
Colony maintenance
ReferencesEur. J. Neurosci., 27(10) 2665-2675 (2008). 18547250
J. Clin. Invest., 124(11) 4773-4780 (2014). 25329695
Science, 309(5744) 2232-2234 (2005). 16195467

Health Report

Examination Date / Room / Rack2024/01/22Room:3-CRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Itpr3inositol 1,4,5-triphosphate receptor 317Itpr3<tm1Kmik>targeted mutation 1, Katsuhiko Mikoshiba

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene17mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Itpr2inositol 1,4,5-triphosphate receptor 26Itpr2<tm1Kmik>targeted mutation 1, Katsuhiko Mikoshiba

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)6nls

Ordering Information

Donor DNAMouse PGK promoter, Escherichia coli neomycin-resistant gene, Mouse PGK pA, SV40 large tumor nuclear location signal, Escherichia coli lacZ gene, Mouse Itpr2 genomic DNA, Mouse Itpr3 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKatsuhiko Mikoshiba (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data