Strain Information | |
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Image | |
BRC No. | RBRC10291 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Itpr2<tm1Kmik> Itpr3<tm1Kmik> |
Former Common name | IP3R2 IP3R3 double knockout mice |
H-2 Haplotype | |
ES Cell line | J1 [129S4/SvJae] |
Background strain | |
Appearance | |
Strain development | Developed by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2005. J1 ES cells derived from 129S4/SvJae were used for Itpr2. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1 were used for Itpr3. C57BL/6J congenic line by crossing with C57BL/6 for 10 generations. |
Strain description | Double KO line for Itpr2 (inositol 1,4,5-trisphosphate receptor 2) and Itpr3 (inositol 1,4,5-triphosphate receptor 3). Each modified allele is derived from RBRC10289 and RBRC10290. |
Colony maintenance | |
References | Science, 309(5744) 2232-2234 (2005). 16195467 Eur. J. Neurosci., 27(10) 2665-2675 (2008). 18547250 J. Clin. Invest., 124(11) 4773-4780 (2014). 25329695 |
Health Report | |
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Examination Date / Room / Rack | 2024/01/22Room:3-CRack:L |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene17mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene6mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)6nls |
Ordering Information | |
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Donor DNA | Mouse PGK promoter, Escherichia coli neomycin-resistant gene, Mouse PGK pA, SV40 large tumor nuclear location signal, Escherichia coli lacZ gene, Mouse Itpr2 genomic DNA, Mouse Itpr3 genomic DNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Katsuhiko Mikoshiba (RIKEN) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |