Strain Data Sheet
RBRC10349
Strain Information | |
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| Image | |
| BRC No. | RBRC10349 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | STOCK Oxct2a<em1Osb> Oxct2b<em1Osb> |
| Former Common name | Oxct2a-1/wt Oxct2b-1722/wt |
| H-2 Haplotype | |
| ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Keisuke Shimada and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2018. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background. |
| Strain description | Oxct2a<em1Osb>; 1 bp deletion at exon 1ctggcagtatcctcctccctgctctgtGACGCAGCGCAGAGGTAATGTGCACTGCTCTGCAAAGGTGAACCTGGCCTTTCTCTCTCTGTCCCGGAGCTGGGAGCCCCGTGGCCGTGGACGATGGCGGCGCTGAGGCTTCTGGCCTGGGCGCTCCCACGTGGGGTATCTGCTCTCCGCCCACCACCCGCGCTGCCCCACCGCCTTATCCGCCGCTATGTTTCGGACCGCAGCGGGAGTGTCCATTTCTACACGGACCCGGTGAAGGCGGTGGAGGGCGTCAAGGACGGGTCTACCGTCATGCTCGGGGGCTTCGGGCTCTGCGGCATCCCCGAGAACCTGATCGGTGCGCTGAAGACCAAGGGCGTGAAGGACCTGAAGATCGTCAGCAGCAACGTGGGCGTGGACGACTTCGGCCTGGGCATCTTGCTGGCCTCCAAGCAGGTCAGGCGCGTGGTGTGCTCCTACCTGGGGGAGAACGCGCTCTGCGAGAAGCTCTACCTGGCGGGCGAGCTGGAACTGGAGATGACGCCGCAGGGAACTTTGGCCGAGCGCATCCGCGCGGGTGGCACTGGCGTGCCCGCCTTCTACACGCCCACAGGCTATGGAACGCTGGTGCAGGAAGGGGGCTCTCCAATCCGGTACGCGCCCGATGGCCACCTGATTACTCTAAGTGAGCCGCGAGAAGTACGCGAGTTCCAGGGCCGCTTCTACCTGCTAGAGCACGCTATCCGCGCTGACTTCGCACTGATCAAGGGCTGGAAGGCCGACCGCTCCGGCAACGTGATCTTCAGGGGCAGCGCGCGCAACTTCAATGTGCCCATGTGCAAGGCCGCGGACATCTCGGTGGTGGAAGTGGAGGAGATCGTGGACGTGGGTACTTTCGCCCCGGAAGACATCCACGTCCCCAACATCTATGTGGACCGCGTGATCAAGGGGCCGAAGTTCGAGAAGCGCATCGAGCGCCTAACCACACGTGACAGCAAGCCCGCGCCCGGGAGCAAAGACAATGACCCTTCCAGGACGCGCATCATCAAGCGCGCGGCTCTGGAGTTCCAGGACGGCATGTATGCCAATCTGGGCATCGGGATCCCTGTCTTGGCGAGCAACTACATCAGCCCCAAGATGACCGTCTACCTGCACAGTGAAAACGGAATCCTGGGCTTGGGCCCGTTCCCTTTGAAAAATGAGGTAGATGCCGACGTCATCAACGCAGGCAAGCAGACAGTGACGGTGGTTCCCGGGGGCTGTTTCTTCGCCAGCGATGACTCTTTTGCCATGATCCGTGGCGGACACCTCCAACTGACCATGCTTGGGGCCATGCAGGTTTCTCAATACGGCGACCTGGCCAACTGGATGGTGCCAGGCAAGAAGGTGAAGGGCATGGGCGGGGCCATGGACTTGGTGTCTAGTAAAAAGACCAGAGTTGTAGTCACCATGGAACACTGCACCAAGACAAAGCAGCCCAAAATCTTGAAGAAATGCACCATGCCATTGACCGGCAAGCGCTGCGTGGACCTCATCATCACTGAGAAGGCAGTGTTTGAAGTGAACCACTCAAAGGGGCTGACACTGGTGGAGCTGTGGGAGGGCTCGTCGGTAGATGACATCAAGGCCACCACAGCCTGTTCATTTGCCGTGTCCCCCAACCTCAAGCCCATGCAGCAGATTAAACTTGATGCTTGAGGAGCCCTCCAGGGCTAATTTGCCAGTTAGTGACAACTGGACATCCTTAGCAGCACCTGACCACTGTGCCACACTGGCTCCTCCAGCTTCCTTCTGCTAGATGGTGTCTATCGAGAGCCATGTGACCTTAATTAAAAACCCTAACTTGAgccagtatgtcctgtccctaaccattgagaactctggtccccctctcccactttctgtgacattcacataccaggctgcagagggttccctgggaggtctcaacaccagtgctgccctcataactgagtcaggaaacatggaggaaaacatgggacaggaaggcatcctacccagagtgcagctggtgctacttcactgcagccaagaaacacagaactgtggatcccggatcgggaggaggagccctccatcttgtccctctgcattgtcttcttgggtcctcgtttctgttcttaattttgtttccctgaaatctgcctgcacccaacttcctcctgggagctcgcgtgtgtccctgtgtttcctgtgggaatctgacttcaggaagatgcttggtgctggcactccacctggaagacccactgtgcactaacagtcccagtgtgtgtaaaggaagaagctgggctcagcacaggtggagtggaatctaaccaatggcatggatagattcttccctggtgaagtgcagtgtcactgttgctgcacctcagtgacactgtcctggaagtcccgttggcctgactgcctgaccccagtgtggcagttctgcgatctgtcattctgaatctgtgagctcgaaactaaagtgaagagtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgcgtgtgtgtgtgcgtgtgtgtgtgcgtgtgtgtgcgtgtgtgcgtgtgtgtgcgtgtgtgtgtgcgtgtgtgtgtgtgtgcgtgtgtgtgtgtgtgcttgtgtgtgtgtgtgtgtgcatgtggggtgtatgtgggtgtacgOxct2b<em1Osb>; 1722 bp deletioncggtgctcactgccatttgtcagcagcattaagcaggggtttgaagtgtctgtccaaggctcttgagagacaggctgggcagcaggaatggccaagggtgtcctcggctgagtgagacccacgaccatccctggcagtgtccacctccctgctctgtgacgcagcgcagagcgctgctctgccaaagtgcacctggtctttCTCTCTCTGTCCCGGAGCTGGGAGCCCGATGGCTGTGGAAGATGGCGGCGCTGAGGCTTCTGGCCTGGGCGCTCCCACGTGGGGTCTCTGCTCTCCGCCCACGACCTGCGCTGCCCCACCGCCTTATCCGCCGCTATGTTTCGGACCGCAGCGGGAGTGTCCATTTCTACACGGACCCGGTGAAGGCGGTGGAGGGCGTCAAGGATGGGTCGACCGTCATGCTCGGGGGCTTCGGGCTCTGCGGCATCCCCGAGAACCTGATCGGTGCGCTGAAGACCAAGGGCGTGAAGGACCTGAAGATCGTCAGCAGCAACGTGGGCGTGGACGACTTCGGCCTGGGCATCTTGCTGGCCTCCAAGCAGGTCAGGCGCGTGGTGTGCTCCTACCTGGGGGAGAACGCGCTCTGCGAGAAGCTCTACCTGGCGGGCGAGCTGGAACTGGAGATGACGCCGCAGGGAACTCTGGCCGAGCGCATCCGCGCGGGTGGCACTGGCGTGCCCGCCTTCTACACGCCCACAGGCTACGGAACGCTGGTGCAGGAAGGGGGCTCTCCAATCCGGTACGCGCCCGATGGCCACCTGATTACTCTAAGTGAGCCGCGAGAAGTACGCGAGTTTCAGGGCCGCTTCTACCTGCTAGAGCACGCTATCCGCGCTGACTTCGCACTGATCAAGGGCTGGAAAGCCGACCGCTCGGGCAACGTGATCTTCAGAGGCAGCGCGCGCAACTTCAATGTGCCCATGTGCAAGGCCGCGGACATCTCGGTGGTAGAAGTGGAGGAGATCGTGGACGTTGGTACTTTCGCCCCGGAAGACATCCACATCCCCAACATCTATGTGGACCGCGTGATCAAGGGGCCGAAGTTCGAGAAGCGCATCGAGCGCCTAACCACACGTGACAGCAAGCCCGCGCCCGGGAGCAAAGACAATGACCCTTCCAGGACGCGCATCATCAAGCGCGCGGCTCTGGAGTTCCAGGACGGCATGTATGCCAATCTGGGCATCGGGATCCCTGTCTTGGCGAGCAACTACATCAGCCCCAAGATGACCGTCTACCTGCACAGTGAAAACGGAATCCTGGGCTTGGGCCCGTTCCCTTTGAAAAATGAGGTAGATGCCGACGTCATCAACGCAGGCAAGCAGACAGTGACGGTGGTTCCCGGGGGCTGTTTCTTCGCCAGCGATGACTCTTTTGCCATGATTCGTGGCGGACACCTCCAACTGACCATGCTTGGGGCCATGCAAGTTTCTCAATACGGCGACCTGGCCAACTGGATGGTGCCAGGCAAGAAGGTGAAGGGCATGGGTGGGGCCATGGACTTGGTGTCTAGTAAAAAGACCAGAGTGGTAGTCACCATGGAACACTGTACCAAGACAAAGCAGCCCAAAATCTTGAAGAAATGCACCATGCCGTTGACTGGCAAGCGCTGCGTGGACCTCATCATCACTGAGAAGGCGGTGTTTGAAGTGAACCACTCAAAGGGGCTGACGCTGGTGGAGCTGTGGGAGGGCTCGTCGGTAGATGACATCAAGGCCACCACAGCCTGTTCATTTGCTGTGTCCCCCAACCTCAAGCCCATGCAGCAGATTAAACTTGATGCTTGAGGAGCCCTCCAGGGCTGATTTTCCAGTTAGTGACAACTGGACATCCTTAGCAGCACCTGACCACTGTGCCACACTGGCTCCTCCGCCTTCCTTCTGCTAGATGGTGTCTAGTGAGAGCCATGTGACCTTAATTAAAAACCCTAACCTGAgcctgtgtgtcctgtccctaaccattgaaaacactgatccccctctcccattttctgtgacattcacataccaggctgcagagggttccctgggagtctcaacaccagtgctgccctcataactgagtcaggaaacatggaggaaaacatgggacaggaaggcatcctacccagagtgcagctggtgctacttcactgcagccacgaaac |
| Colony maintenance | |
| References | Biol. Reprod., 101(2):501-511 (2019). 31201419 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Oxct2a3-oxoacid CoA transferase 2A4Oxct2a<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Oxct2b3-oxoacid CoA transferase 2B4Oxct2b<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
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| Donor DNA | human hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Mouse a part of Oxct2b gene |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 101(2):501-511 (2019).The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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