Strain Information | |
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Image | |
BRC No. | RBRC10392 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Ebf3<tm1(cre/ERT2)Tng> |
Former Common name | Ebf3-CreERT2 |
H-2 Haplotype | |
ES Cell line | KY1.1[(C57BL/6J x 129S6/SvEvTac)F1] |
Background strain | C57BL/6NCrSlc |
Appearance | |
Strain development | Developed by Masanari Seike, Osaka University in 2016. KY1.1(129/B6) ES cells were used, and then mice were backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic strain. |
Strain description | Ebf3-CreERT knock-in mice. These mice have a tamoxifen-inducible cre (CreERT2) at the Ebf3 locus. IRES-CreERT2 and FRT-PGKpro-neo-pA-FRT cassettes were inserted into the 3' UTR of exon 16 of the Ebf3 locus. |
Colony maintenance | |
References | Genes Dev., 32, 359-372 (2018). 29563184 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)7ERT2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)7 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase7cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | Encephalomyocarditis virus Internal ribosomal entry site, Bacteriophage P1 Cre, Human ERT2 cDNA, Yeast Frt sites, Mouse PGK promoter, E. coli Neomycin resistant gene, Mouse PolyA signal, mouse Ebf3 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takashi Nagasawa) and enter into a Material Transfer Agreement with Osaka University. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Takashi Nagasawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |