Strain Data Sheet

RBRC10443

Strain Information

Image
BRC No.RBRC10443
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(Clgn-Izumo1/EYFP1-155<*>F46L/V150A/M153T, Clgn-Izumo1/EYFP156-239<*>A206K)1Nino
Former Common nameIzumo1-BiFC TG
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Naokazu Inoue, Fukushima Medical University in 2018. B6D2 genetic background.
Strain descriptionTransgenic mice expressing Izumo1 proteins fused with EYFP (1-155) and EYFP (156-239) under the control of Clgn gene promoter. Both EYFP fragments are separately fused with Izumo1 proteins.
Colony maintenance
ReferencesCell Cycle, 17(11):1279-1285 (2018). 29954238

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyAUNHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Izumo1izumo sperm-egg fusion 1UNIzumo1mouse Clgn promoter

Ordering Information

Donor DNAmouse Calmegin promoter, mouse Izumo1 cDNA, jellyfish Eyfp<*> F46L/V150A/M153T/A206K cDNA, rabbit beta-globin polyA signal
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorNaokazu Inoue (Fukushima Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data