Strain Data Sheet
RBRC10465
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10465 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | STOCK Amy1<em1(luc/mVenus)Matam> |
| Former Common name | Amy1 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Naokazu Inoue and Masayuki Sekimata, Fukushima Medical University, and Akiko Sekimata, Yamagata University in 2018. This strain was generated by the CRISPR/Cas9 using fertilized eggs derived from B6D2F1. Mice were further crossed with ICR. Mixed genetic background. |
| Strain description | Reporter mouse strain which can monitor Amylase 1 (Amy1) secretion with the NanoLuc (Nluc) and Amy1 expressing cells with mVenus. A DNA construct, Nluc-3xFLAG-T2A-mVenus-BGHpA, was knocked in at downstream of the Amy gene promoter. |
| Colony maintenance | |
| References | |
Health Report | |
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| Examination Date / Room / Rack | 2022/06/13Room:3-5Rack:C |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAG3xFLAG tag (synthetic)3FLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA3GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter NanoLucluciferase (Oplophorus gracilirostris)3NanoLuc Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter T2AThosea asigna virus 2A peptide (insect)3T2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mVenusYellow Fluorescent Protein (Aequorea victoria)3mVenus |
Ordering Information | |
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| Donor DNA | Oplophorus gracilirostris NanoLuc cDNA, synthetic FLAG (3xFLAG tag), Thoseaasigna virus 2A peptide (T2A), Aequorea victoria mVenus (variant of enhanced yellow fluorescent protein) cDNA, Bos taurus GH (Growth hormone polyA), mouse Amy1 genomic DNA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | Masayuki Sekimata (Fukushima Medical University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |