Strain Data Sheet

RBRC10468

Strain Information

Image
BRC No.RBRC10468
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Scn2a<tm2.1Kzy>
Former Common nameScn2a-floxed, Scn2afl/+
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Ikuo Ogiwara and Kazuhiro Yamakawa, RIKEN BSI in 2010. E14 ES cells(129P2/OlaHsd) were used and the Neo gene was deleted by CAG-Flpe mice. C57BL/6J congenic line (24 generations).
Strain descriptionScn2a (sodium channel, voltage-gated, type II, alpha) floxed mice. Exon 2 is flanked by a pair of loxP.
Colony maintenance
ReferencesCommun. Biol., 1, Article number: 96 (2018). 30175250

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Scn2asodium channel, voltage-gated, type II, alpha2Scn2a<tm2.1Kzy>targeted mutation 2.1, Kazuhiro YamakawaScn2a1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Scn2a genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Ogiwara, et al., (2018) Communications Biology volume 1, Article number: 96. https://doi.org/10.1038/s42003-018-0099-2.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKazuhiro Yamakawa (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data