Strain Information | |
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Image | |
BRC No. | RBRC10469 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Scn2a<tm1.1Kzy> |
Former Common name | Scn2a-knock-in, Scn2aRX/+ |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Ikuo Ogiwara and Kazuhiro Yamakawa, RIKEN BSI in 2007. E14 ES cells(129P2/OlaHsd) were used and the Neo gene was deleted by CAG-Flpe mice. C57BL/6J congenic line (17 generations). |
Strain description | A strain harboring a point mutation (R102*) at the Scn2a (sodium channel, voltage-gated, type II, alpha) gene. Homozygous mutants are lethal neonatally. |
Colony maintenance | |
References | Commun. Biol., 1, Article number: 96 (2018). 30175250 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Scn2asodium channel, voltage-gated, type II, alpha2Scn2a<tm1.1Kzy>targeted mutation 1.1, Kazuhiro YamakawaScn2a1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP |
Ordering Information | |
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Donor DNA | Phage P1 loxP site, S.cerevisiae Frt site, mouse Scn2a genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Ogiwara, et al., (2018) Communications Biologyvolume 1, Article number: 96. https://doi.org/10.1038/s42003-018-0099-2In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Kazuhiro Yamakawa (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |