Strain Information | |
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Image | |
BRC No. | RBRC10581 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6N-Sem1<tm1.2Smta> |
Former Common name | Sem l f/f |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | |
Appearance | |
Strain development | Developed by Jun Hamazaki, The University of Tokyo in 2011. RENKA ES cells derived from C57BL/6N were used. Mice were further backcrossed to C57BL/6N for more than 6 generations. |
Strain description | Sem1 (SEM1, 26S proteasome complex subunit) floxed mice. A pair of loxP flanks exon 2 of the Sem1 gene. An FRT-flanked Neo gene was deleted by crossing with a Flp-mouse strain. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP |
Ordering Information | |
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Donor DNA | Phage P1 1oxP sites, Yeast FRT (flippase recombination target) site, mouse Sem1 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Shigeo Murata (The University of Tokyo) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |