Strain Information | |
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Image | |
BRC No. | RBRC10586 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | B6.Cg-Chil3<em1(Venus)Mtka> |
Former Common name | Ym1-Venus |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Masato Tanaka at Tokyo University of Pharmacy and Life Sciences and Masaki Ohmuraya at Kumamoto University in 2014. This strain was generated using KTPU8 ES cells derived from (C57BL/6 x CBA)F1 and mice were backcrossed to C67BL/6J for more than 10 generations. |
Strain description | Knockin mice in which Venus is inserted at the translation initiation codon of the Chil3 (Ym1) gene. |
Colony maintenance | |
References | Sci. Immunol., 3(28)(2018). 30291130 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyA3HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)3Venus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene3mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter |
Ordering Information | |
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Donor DNA | jellyfish Venus cDNA, Rabbit beta-globin polyA, Saccharomyces cerevisiae FRT sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli Neomycin resistant gene, mouse Chil3 genomic DNA |
Research application | FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Ikeda et al. 2018 Science Immunology (PMID: 30291130, DOI: 10.1126/sciimmunol.aat0207.)The RECIPIENT shall not distribute the biological resource to others without a written consent from the depositor. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER (Masato Tanaka and Gen Nishitai). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Masato Tanaka (Tokyo University of Pharmacy and Life Sciences) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |