Strain Data Sheet

RBRC10624

Strain Information

Image
BRC No.RBRC10624
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tlk2<tm1.1Chum>
Former Common nameTlk2 gene targeted knockout
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinichiro Chuma, Institute for Frontier Life and Medical Sciences, Kyoto University in 2019. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. The strain was backcrossed to C57BL/6J for 11 generations.
Strain descriptionTlk2 knockout mice. Exon 14 to Exon 16 were deleted.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tlk2tousled-like kinase 2 (Arabidopsis)11Tlk2<tm1.1Chum>targeted mutation 1.1, Shinichiro Chuma

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Ordering Information

Donor DNAP1 Phage loxP site, mouse Tlk2 genomic DNA
Research applicationCre/loxP system
Specific Term and Conditions・ Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval form the
DEVELOPER (Dr. Shinichiro Chuma) using the Approval Form.
・ The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a non-for profit
organization for a research and academic research purpose.
DepositorShinichiro Chuma (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data