Strain Data Sheet

RBRC10628

Strain Information

Image
BRC No.RBRC10628
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tgfbr2<tm1(cre/Esr1<*>)Sleep>
Former Common nameTgfbr2-CreERT2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. ES cell line derived from C57BL/6J was used. C57BL/6J genetic background.
Strain descriptionKnockin mice in which Cre/ERT2 was inserted at the translation initiation codon of the Tgfbr2 gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tgfbr2transforming growth factor, beta receptor II9Tgfbr2<tm1(cre/Esr1<*>)Sleep>targeted mutation, Yu Hayashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbchicken Beta-actin polyA9Actb

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Esr1*human Estrogen receptor9Esr1*

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide9P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase9cre

Ordering Information

Donor DNAP2A (porcine teschovirus-1 2A peptide), Bacteriophage P1 Cre recombinase, Homo sapiens Estrogen receptor cDNA, chicken Beta-actin polyA, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli Neomycin resistance gene, mouse Tgfbr2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorYu Hayashi (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data