Strain Data Sheet

RBRC10640

Strain Information

Image
BRC No.RBRC10640
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(tetO-Flag/Kcnj2,-NLS/lacZ)321Sleep
Former Common nametetO-Flag-Kir2.1-NLS-lacZ Tg321
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice (line 321) expressing Flag-Kir2.1 and NLS-lacZ under the control of tetO promoter.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kcnj2potassium inwardly-rectifying channel, subfamily J, member 2UNKcnj2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 Intron-polyAUN

Ordering Information

Donor DNAMus musculus Kir2.1, Escherichia coli tetO, Nuclear Localization Signal(NLS), Escherichia coli lacZ, SV40 Intron-polyA
Research applicationFluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorYu Hayashi (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data