Strain Data Sheet

RBRC10642

Strain Information

Image
BRC No.RBRC10642
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Slc17a6-neo,-tTA)305Sleep
Former Common nameVglut2-loxP-stop-loxP-tTA Tg305
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A BAC transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice (line 305) expressing tTA under the control of Vglut2 promoter and Cre recombinase.
Colony maintenance
ReferencesScience. 2015 Nov 20;350(6263):957-61. 26494173

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 Intron-polyAUN

Ordering Information

Donor DNAMus musculus Slc17a6 (Vglut2), Bacteriophage P1 loxP, phage T7 gb2 promoter, Escherichia coli Neomycin resistance, Escherichia coli tTA, SV40 Intron-polyA
Research applicationCre/loxP system
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hayashi et al., Science. 2015 Nov 20;350(6263):957-61.
DepositorYu Hayashi (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data