Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC10696  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129P2-Aplf<tm1Okad>  
Former Common name Aplf KO  
H-2 Haplotype No Data  
ES cell line E14K [129P2/OlaHsd]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development B6.129P2-Aplf<tm1Okad> has been established by H Okada. ES cell: 129/Ola
Backcrossed to C57BL/6J including B6 male more than 10 times.
Aplf-/- mice are viable with no gross abonormality and grow normally. Both male and female Aplf-/- mice are fertile. We have maintained the mouse colony by backcrossing Aplf+/- with B6J since previous reports suggested a functional link between Aplf and telomere function; however, several crosses between homozygotes did not affect fertility. Cellularity of B cell was slightly lower than wild-type mice. Aplf-/- mice showed moderately impaired C-NHEJ activity. Thus, irradiated mutant mice showed higher rates of p53-dependent cell death and fewer chromosomal translocations.  
Strain description Aprataxin and PNKP-like factor (APLF, also referred to as Xip1, C2orf13, and PALF) is a poly(ADP-ribose) or PAR-binding protein that interacts with C-NHEJ repair factors, XRCC4-DNA ligase 4 and Ku, to facilitate C-NHEJ in a PAR polymerase 3 (PARP3)-dependent manner. APLF can undergo ATM- and PARP3-dependent phosphorylation at serine-116 following ionizing radiation (IR), which is critical for the recruitment of APLF to the sites of DNA lesion to resolve γH2AX DNA damage signals. Accumulation of APLF to DSBs has been demonstrated to promote the retention of XRCC4/DNA ligase 4 complex in chromatin to facilitate DNA ligation during C-NHEJ. Furthermore, a phospho-ablative mutant of APLF (APLFS116A), which disabled IR-induced phosphoryation at serine-116 of APLF, exhibited higher persistent γH2AX DNA damage signal and lower cellular survival in colony formation assays after IR exposure reminiscent of cells with APLF depletion. These data suggest that APLF works downstream of ATM and PARP3 to modulate C-NHEJ after IR treatment. A moderate reduction of C-NHEJ activity by the depletion of APLF impedes radiation-induced oncogenic translocation in normal tissues and malignancy-associated mortality.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Aplf  
Symbol name aprataxin and PNKP like factor  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.  
Additional information
 1 No Data  
Depositor Okada, Hitoshi (Kindai University)  Okada, Hitoshi 
Strain Status /
Availability
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