Strain Data Sheet

RBRC10711

Strain Information

Image
BRC No.RBRC10711
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Ntng1<tm1(lacZ)Ito>
Former Common nameNtng1-KO(+LacZ)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Shigeyoshi Itohara, RIKEN Brain Science Institute. E14 ES cells derived from 129P2/ola were used.
Strain descriptionNtng1 (netrin G1) knockout mice. N-LacZ was placed in frame to ATG codon with deletion of 246 bp downstream.
Colony maintenance
ReferencesJ. Neurosci., 34(47) 15779-92 (2014). 25411505
Proc. Natl. Acad. Sci. USA, 104(37):14801-6 (2007). 17785411

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ntng1netrin G13Ntng1<tm1(lacZ)Ito>targeted mutation 1, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)3nls

Ordering Information

Donor DNASV40 Nuclear laocalozation signal(NLS), E.coli LacZ, pA, P1 phage loxP site, mouse Ntng1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA. 104(37):14801-6 (2007).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data