Strain Data Sheet

RBRC10729

Strain Information

Image
BRC No.RBRC10729
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Lrrc4<tm1(lacZ)Ito>
Former Common nameNgl2-KO
H-2 Haplotype
ES Cell lineMS12 [C57BL/6]
Background strain
Appearance
Strain developmentDeposited by Shigeyoshi Itohara, RIKEN Center for Brain Science. MS12 ES cells derived from C57BL6, and then mice were crossed to C57BL/6J.
Strain descriptionNgl2 (Lrrc4) coding and its downstream are replaced with N-LacZ and loxP.
Colony maintenance
ReferencesJ. Neurosci., 34(47):15779-92 (2014). 25411505

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lrrc4leucine rich repeat containing 46Lrrc4<tm1(lacZ)Ito>targeted mutation 1, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)6nls

Ordering Information

Donor DNAマウス NGL2、pgk, P1ファージ LoxP配列, SV40 Nuclear localization signal(NLS), E.coli LacZ、neo耐性遺伝子
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 34(47):15779-92 (2014).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data