Strain Data Sheet

RBRC10745

Strain Information

Image
BRC No.RBRC10745
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(tetO-YC2.60)#Ito
Former Common nameTRE-YC2.60
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shigeyoshi Itohara at RIKEN.
Strain descriptionTrasgenic mice expressing YC2.60 under the control of teto promoter.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/01/09Room:3-BRack:B
2023/10/10Room:3-BRack:B
2023/07/10Room:3-BRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
YC2.60Yellow Cameleon 2.60 (Aequorea victoria ECFP cDNA, Xenopus laevis calm2 cDNA, calmodulin (CaM)-binding peptide (rabbit skeletal muscle myosin light chain kinase (MLCK) cDNA), circularly permuted form of the Venus yellow fluorescent protein cDNA)UNYC2.60E. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 Intron polyAUN

Ordering Information

Donor DNAE. coli tetO (tetracycline operator), Yellow Cameleon 2.60 (Aequorea victoria ECFP cDNA, Xenopus laevis calm2 cDNA, calmodulin (CaM)-binding peptide (rabbit skeletal muscle myosin light chain kinase (MLCK) cDNA), circularly permuted form of the Venus yellow fluorescent protein cDNA), Woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), SV40 Intron polyA
Research applicationFluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
Nat. Biotechnol., 20, 87-90 (2002).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the YC2.60 using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Sequence of Y2.60
Genotyping protocol -PCR-

BRC mice in Publications

No Data