Strain Data Sheet

RBRC10748

Strain Information

Image
BRC No.RBRC10748
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Rapgef4<tm1Ito>
Former Common nameCGII-flox
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shigeyoshi Itohara at RIKEN.
Strain descriptionFloxed Rapgef4 mice. Frt-flanked pgk-neo cassettes was placed at downstream of Exon 3. A pair of loxP flanks Exon 3 and pgk-neo cassette.
Colony maintenance
ReferencesMol. Brain, 8:38 (2015). 26104314

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rapgef4Rap guanine nucleotide exchange factor (GEF) 42Rapgef4<tm1Ito>targeted mutation 1, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAマウス cAMP-GEFII、pgk, P1ファージ LoxP, Yeast FRT, E.coli neo
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Brain, 8:38 (2015).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data