Strain Data Sheet

RBRC10750

Strain Information

Image
BRC No.RBRC10750
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Lrrc4<tm3(KOr)Ito>
Former Common nameNgl2-kusabiraorange
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masataka Fujita and Shigeyoshi Itohara at RIKEN. CMTI ES cells were used. Mice were maintained by crossing to C57BL/6J. C57BL/6 genetic background.
Strain descriptionKnockin strain which replaces intracellular domain of Ngl2 with mKusabiraOrange.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lrrc4leucine rich repeat containing 46Lrrc4<tm3(KOr)Ito>targeted mutation 3, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
KOOrange Fluorescent Protein, Kusabira Orange (Verrillofungia concinna)6KO

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Ordering Information

Donor DNAマウス Ngl2, pgk, 出芽酵母 FRT配列, E.coli Neo耐性遺伝子, phage T7 gb2 promoter, サンゴ類 Kusabira-orange
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data