Strain Data Sheet

RBRC10760

Strain Information

Image
BRC No.RBRC10760
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Gsdma<tm1Tshir>/Ms
Former Common nameGsdmaLacZ
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Toshihiko Shiroishi and Shigekazu Tanaka, National Institute of Genetics in 2009. TT2 ES cells derived from (C57BL/6 x CBA)F1 were used. Mice were backcrossed to C57BL/6J for more than 10 generations. C57BL/6 congenic line.
Strain descriptionKnock-in mice for LacZ reporter at the translation initiation site of the Gsdma (gasdermin A) gene. Frt-flanked neo gene with a loxP at 3’ side was placed at intron 3. Another loxP was placed at intron 1.
Colony maintenance
ReferencesCarcinogenesis, 44(4):341-349 (2023). 36753047
G3 (Bethesda), 3(10):1843-50 (2013). 23979942

Health Report

Examination Date / Room / Rack2023/11/27Room:4-CRack:I
2023/08/28Room:4-CRack:I
2023/05/29Room:4-CRack:I
2023/02/27Room:4-CRack:I
2022/11/29Room:4-CRack:I
2022/08/29Room:4-CRack:I
2022/05/31Room:4-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gsdmagasdermin A11Gsdma<tm1Tshir>targeted mutation 1, Toshihiko Shiroishi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal11

Ordering Information

Donor DNAP1 bacteriophage loxP sites, E. coli LacZ, SV40 polyA signal, S. cerevisiae Frt sites, Mouse PGK promoter, E. coli Neomycin registant gene, mouse Gsdma genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. G3 (Bethesda), 3(10):1843-50 (2013).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. (Contact info: Tsuyoshi Koide, Ph.D. <tkoide@nig.ac.jp>, Mouse Genomics Resource Laboratory)
DepositorToshihiko Shiroishi (National Institute of Genetics)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Mouse of the Month Mar 2020
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data