Strain Information | |
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Image | |
BRC No. | RBRC10760 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.B6CB-Gsdma<tm1Tshir>/Ms |
Former Common name | GsdmaLacZ |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Toshihiko Shiroishi and Shigekazu Tanaka, National Institute of Genetics in 2009. TT2 ES cells derived from (C57BL/6 x CBA)F1 were used. Mice were backcrossed to C57BL/6J for more than 10 generations. C57BL/6 congenic line. |
Strain description | Knock-in mice for LacZ reporter at the translation initiation site of the Gsdma (gasdermin A) gene. Frt-flanked neo gene with a loxP at 3’ side was placed at intron 3. Another loxP was placed at intron 1. |
Colony maintenance | |
References | G3 (Bethesda), 3(10):1843-50 (2013). 23979942 Carcinogenesis, 44(4):341-349 (2023). 36753047 |
Health Report | |
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Examination Date / Room / Rack | 2023/11/27Room:4-CRack:I 2023/08/28Room:4-CRack:I 2023/05/29Room:4-CRack:I 2023/02/27Room:4-CRack:I 2022/11/29Room:4-CRack:I 2022/08/29Room:4-CRack:I 2022/05/31Room:4-CRack:I |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)11 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal11 |
Ordering Information | |
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Donor DNA | P1 bacteriophage loxP sites, E. coli LacZ, SV40 polyA signal, S. cerevisiae Frt sites, Mouse PGK promoter, E. coli Neomycin registant gene, mouse Gsdma genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. G3 (Bethesda), 3(10):1843-50 (2013).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. (Contact info: Tsuyoshi Koide, Ph.D. <tkoide@nig.ac.jp>, Mouse Genomics Resource Laboratory) |
Depositor | Toshihiko Shiroishi (National Institute of Genetics) |
Strain Status | Live mice Frozen embryos |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Mar 2020 |
BRC mice in Publications |
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No Data |