Strain Data Sheet
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC01105  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Sema3a<tm1.3Tyag>/TyagRbrc  
Former Common name semaphorin III/D (lacZ) Targeting mice  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line  
Background strain C57BL/6NCrlCrlj  
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Masahiko Taniguchi and Takeshi Yagi, National Institute for Physiological Sciences in 1997. TT2 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 5 times.  
Strain description Semaphorin III/D (Sema3a) knockout mice (lacZ reporter). Most of homozygous mice in C57BL/6 genetic background are lethal. About one-tenth of the homozygous mutant mice of ICR background survived (Depositor, personal communication).  
Colony maintenance Heterozygote x Wild-type [or Crossing to C57BL/6NCrlCrlj]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Sema3a  
Symbol name semaphorin III  
Chromosome 5  
Common name coll-1, collapsin-1, Hsema-I, sema III, SEMA1, Semad, semaphorin III, SemD, SemD  
Symbol description No Data  
 2 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 5  
Common name No Data  
Symbol description E. coli beta galactosidase  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 5  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 5  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 5 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 5  
Common name neo; neomycin;  
Symbol description No Data  
References Neuron. 1997 Sep;19(3):519-30.  
Disruption of semaphorin III/D gene causes severe abnormality in peripheral nerve projection.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System,
Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neuron, 19, 519-530 (1997).
Any patent rights and other intellectual properties are retained by the depositor. Users have to contact the depositor in the case of application for any patents with the result from these mice.
When users releases research results that obtained in two years after deposition, users should contact a bailor about collaboration.  
Additional information
 1 Lab HP (Japanese)  
 2 Mouse of the Month Aug 2006  
 3 Genotyping protocol <PCR>  
Depositor Yagi, Takeshi (National Institutes of Natural Sciences)  Yagi, Takeshi 
Strain Status /
(Expected delivery)

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