Strain Data Sheet
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Data update: Apr 19, 2019
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RBRC No. RBRC01308  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Hbegf<tm1Mek>/MekRbrc  
Former Common name HB-EGF lox Knock-in mice(BL6) (HBlox#80/B6)  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Donor strain C57BL/6J  
Background strain C57BL/6J  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Dr. Eisuke Mekada, Osaka University in 2003.  
Strain description B6.Cg-Hbegf<tm1Mek>. Hbegf, heparin-binding EGF like growth factor is a member of the EGF family of growth factor. Hbegf binds to and activates EGF receptor. The targeting vector containing WT HB-EGF cDNA flanked by loxP sites linked to lacZ reporter gene was transfered into TT2 ES cells.  
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJmsSlc)  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Hbegf  
Symbol name heparin-binding epidermal growth factor-like growth factor  
Chromosome 18  
Common name No Data  
Symbol description No Data  
 2 Symbol Hbegf  
Symbol name heparin-binding epidermal growth factor-like growth factor (mouse)  
Chromosome 18  
Common name No Data  
Symbol description No Data  
 3 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 18  
Common name No Data  
Symbol description E. coli beta galactosidase  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 18  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 5 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 18  
Common name neo; neomycin;  
Symbol description No Data  
References Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3221-6. Epub 2003 Mar 5.  
Heparin-binding EGF-like growth factor and ErbB signaling is essential for heart function.
Research applications Cardiovascular Research,
Cell Biology Research,
Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.  
Additional information
 1 Lab HP (Japanese)  
 2 Mouse of the Month Apr 2006  
 3 Genotyping protocol <PCR>  
Depositor Mekada, Eisuke (Osaka University)  Mekada, Eisuke 
Strain Status /
Availability
(Expected delivery)

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