Strain Data Sheet
RBRC02594
Strain Information | |
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| Image | |
| BRC No. | RBRC02594 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Sept3<tm1Keng>/SiaRbrc |
| Former Common name | Sept3(-) (Acc. No. CDB0422K) |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Mineko Kengaku, RIKEN Brain Science Institute. A neomycin resistance cassette flanked by loxP sites was transferred into TT2 ES cells to replace a partial region of exon 2 and exons 3-5 of Sept3 gene. |
| Strain description | Sept3 knock-out mice. Septins are a family of GTPase proteins that are required for cytokinesis in most eukaryotes. Sept3 is a brain-specific protein that is abundant in synaptic terminals. Sept3 knockout mutant mice show normal histogenesis and neuronal differentiation. |
| Colony maintenance | |
| References | J. Neurochem., 102, 77-92 (2007) 17564677 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)15neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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| Donor DNA | Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Sept3 genomic DNA |
| Research application | Cre/loxP system Neurobiology Research |
| Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from Dr. Mineko KENGAKU (kengaku@brain.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0422K and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, J Neurochem. 2007 Jul; 102 (1): 77-92. (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
| Depositor | RIKEN CDB (RIKEN CDB) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Genetic Background |
BRC mice in Publications |
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| No Data |