Strain Information | |
---|---|
Image | |
BRC No. | RBRC05658 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Siglech<tm1.1Ksat> |
Former Common name | Siglech<dtr>, B6-Siglech/DT-GFP KI |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2009. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. The mutant mice were backcross to C57BL/6J. |
Strain description | IRES-DTR-EGFP cassette were inserted in to 3’ untranslated region of Siglech, Siglech sialic acid binding Ig-like lectin H gene. Plasmacytoid dendritic cells (pDCs) from homozygous mutant mice are defect in the transcriptional and cell surface expression of Siglech. In mice with diphtheria toxin (DT) induced specific ablation of pDCs. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)] |
References | J. Allergy Clin. Immunol., (2018). 29477579 Immunity, 35, 958-971 (2011). 22177923 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)7EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBEGFheparin-binding EGF-like growth factor (human)7HBEGF Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRES2internal ribosomal entry site (EMCV)7IRES2internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP |
Ordering Information | |
---|---|
Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site 2 (ires2), human diphtheria toxin receptor (DTR) corresponds to the heparin binding EGF-like growth factor, Aequorea Victoria EGFP, Phage P1 loxP site, mouse Siglech genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System Immunology and Inflammation Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 35, 958-971 (2011). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a “MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes" specified by the DEPOSITOR and Inserm. |
Depositor | Katsuaki Sato (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:Depositor's present address:Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki. |
BRC mice in Publications |
---|
Konishi H, Okamoto T, Hara Y, Komine O, Tamada H, Maeda M, Osako F, Kobayashi M, Nishiyama A, Kataoka Y, Takai T, Udagawa N, Jung S, Ozato K, Tamura T, Tsuda M, Yamanaka K, Ogi T, Sato K, Kiyama H. Astrocytic phagocytosis is a compensatory mechanism for microglial dysfunction. EMBO J 39(22) e104464(2020) 32959911 Koda Y, Nakamoto N, Chu PS, Ugamura A, Mikami Y, Teratani T, Tsujikawa H, Shiba S, Taniki N, Sujino T, Miyamoto K, Suzuki T, Yamaguchi A, Morikawa R, Sato K, Sakamoto M, Yoshimoto T, Kanai T. Plasmacytoid dendritic cells protect against immune-mediated acute liver injury via IL-35. J Clin Invest 129(8) 3201-3213(2019) 31264967 Li C, Konishi H, Nishiwaki K, Sato K, Miyata T, Kiyama H. A mouse model of microglia-specific ablation in the embryonic central nervous system. Neurosci Res 173 54-61(2021) 34157360 |