Strain Information | |
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Image | |
BRC No. | RBRC06469 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Frem1<tm1Ksek> |
Former Common name | QBRICK KO, B6-Frem1 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Kiyotoshi Sekiguchi, Institute for Protein Research, Osaka University in 2006. 129 derived ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6. |
Strain description | QBRICK (Frem1) gene knockout mice. Exon 2 was replaced with a neo cassette. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6J] |
References | Proc. Natl. Acad. Sci. USA, 103, 11981-11986 (2006). 16880404 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frem1Fras1 related extracellular matrix protein 14Frem1<tm1Ksek>targeted mutation 1, Kiyotoshi Sekiguchi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 PolyA signal4 |
Ordering Information | |
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Donor DNA | Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Frem1 genomic DNA |
Research application | Developmental Biology Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 103, 11981-11986 (2006).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Kiyotoshi Sekiguchi (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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