Strain Data Sheet

RBRC06469

Strain Information

Image
BRC No.RBRC06469
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Frem1<tm1Ksek>
Former Common nameQBRICK KO, B6-Frem1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kiyotoshi Sekiguchi, Institute for Protein Research, Osaka University in 2006. 129 derived ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6.
Strain descriptionQBRICK (Frem1) gene knockout mice. Exon 2 was replaced with a neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J]
ReferencesProc. Natl. Acad. Sci. USA, 103, 11981-11986 (2006). 16880404

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frem1Fras1 related extracellular matrix protein 14Frem1<tm1Ksek>targeted mutation 1, Kiyotoshi Sekiguchi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal4

Ordering Information

Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Frem1 genomic DNA
Research applicationDevelopmental Biology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 103, 11981-11986 (2006).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorKiyotoshi Sekiguchi (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data