Strain Information | |
---|---|
Image | |
BRC No. | RBRC09241 |
Type | Transgene Congenic and Spontaneous Mutation |
Species | Mus musculus |
Strain name | HR1.Cg-Hr<hr> Tg(EF1A-PDHL/roGFP1)121Soht |
Former Common name | Mito-roGFP(MROH121), HR1-hr/Mito-roGFP |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Alexander M. Wolf, Nippon Medical School in 2012. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mutant mice were backcrossed to Hos:HR-1 over 10 generations. |
Strain description | Transgenic mice expressing ratiometric redox-sensitive GFP (roGFP) in the mitochondria of several tissues, including skin epidermal keratinocytes. Hairless mice background. |
Colony maintenance | Tg: Carrier x Noncarrier; hr: Homozygote x Homozygote [or Crossing to Hos:HR-1] |
References | J. Invest. Dermatol., 134(6), 1701-1709 (2014). 24129062 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter CSF3human G-CSF cDNA PolyA signalUNCSF3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter PDHA1mitchondrial targeting seaquence (human pyruvate dehydrogenase E1 subunit leader sequence)UNPDHA1human elongation factor 1 alpha (EF-1 alpha) promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roGFPRedox-sensitive Green Fluorescent Protein (Aequorea victoria)UNroGFP |
Ordering Information | |
---|---|
Donor DNA | human EF1A promoter, mitchondrial targeting seaquence (human pyruvate dehydrogenase E1 subunit leader sequence, PDHA1), jellyfish GFP(EGFP) cDNA, human G-CSF cDNA PolyA signal |
Research application | Dermatology Research Fluorescent Proteins/lacZ System Metabolism Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Invest. Dermatol.,134(6), 1701-1709 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Shigeo Ohta (Nippon Medical School) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |