Strain Information | |
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Image | |
BRC No. | RBRC09421 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | FVB.129S6-Gt(ROSA)26Sor<tm1(Pik3ca*H1047R)Egan> |
Former Common name | R26-Pik3ca(H1047R) |
H-2 Haplotype | |
ES Cell line | |
Background strain | FVB/NJcl |
Appearance | |
Strain development | W4 mESC were transformed with linearized pROSA-Pik3ca-H1047R or pROSA-Pik3ca-E545K plasmid; this plasmid was built from pBIGT and pROSA gene targeting vectors and contained the mouse Pik3ca cDNA with E545K point mutation |
Strain description | Some mice develop blood vessel lesions. |
Colony maintenance | |
References | Cancer Res., 1, 2706-2717 (2011). 21324922 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Pik3ca*H1047R)Egan>targeted mutation 1, Sean E Egan Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)6SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tpAtrimer of the SV40 polyadenylation sequence (tpA)6tpA |
Ordering Information | |
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Donor DNA | adenovirus splice acceptor (SA), Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, trimer of the SV40 polyadenylation sequence (tpA), mouse Pik3ca H1047R cDNA, bovine growth hormone polyadenylation sequence (bpA), mouse Gt(ROSA)26Sor genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must prior contact with the developer scientist(Dr. Sean E. Egan, segan@sickkids.ca, Hospital for Sick Children) and must obtain a use license. |
Depositor | Yasushi Okazaki (Saitama Medical University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |