Strain Information | |
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Image | |
BRC No. | RBRC09646 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Rftn2<tm1b(EUCOMM)Mmtm> |
Former Common name | Raftlin2 KO |
H-2 Haplotype | |
ES Cell line | EGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Misako Matsumoto, Hokkaido University in 2013. A FRT-flanked neo cassette was removed by a CAG-Cre line. C57BL/6 background. |
Strain description | Rftn2 KO mice. Exon 2 of the Rftn2 gene was deleted. |
Colony maintenance | |
References | J. Immunol., 196(9):3865-76 (2016) 27022195 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)1En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site1Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP1loxP |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) site, Mouse En2 intron 2/exon 3 splice acceptor sequence, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, Bacteriophage P1 loxP site, mouse Rftn2 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 196(9):3865-76 (2016).The RECIPIENT shall acknowledge the EUCOMM as the provider of the knockout vector. |
Depositor | Misako Matsumoto (Hokkaido University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |