Strain Data Sheet

RBRC09658

Strain Information

Image
BRC No.RBRC09658
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1(CAG-cat,-AKR1C3)Watk>
Former Common namehPGFS KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Kikuko Watanabe, Koshien University in 2015. RENKA ES cell line (C57BL/6N) was used. Mice were maintained in C57BL/6 background.
Strain descriptionKnock-in mice for Cre-dependent overexpression of human AKR1C3. Targeted insertion of CAG-loxP-CAT-FRT-PGK-neo-FRT-loxP-FAM213B-pA cassette into the mouse Rosa26 locus.
Colony maintenance
ReferencesGenes Dev., 5, 1513-1523 (1991). 1653172
Prostaglandins Other Lipid Mediat., 68-69:401-407 (2002). 12432932
FEBS Lett., 462, 335-340 (1999). 10622721

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-cat,-AKR1C3)Watk>targeted mutation 1, Kikuko Watanabe

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
AKR1C3human ortholog mouse Akr1c18 (MGI: 2145420), aldo-keto reductase family 1, member C186AKR1C3CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Ordering Information

Donor DNAマウス(Mus musculus)　ROSA26, ヒト(Homo Sapiens) hPGFS,ウシ(Bos taurus) bovine growth hormone polyA signal, サイトメガロウィルス(cytomegalovirus) cytomegalovirus　enhancer, ニワトリ(Gallus gallus)β-actin promoter, ウサギ(Oryctolagus cuniculus)　β-globin exon, intron, ファージ　P1 (Enterobacteria phage P1) Lox-P, 大腸菌(Escherichia coli)CAT((n) chloramphenicol O-acetyltransferase), SV40(simian virus 40)SV40 polyA, 出芽酵母(Saccharomyces cerevisiae)FRT, マウス(Mus musculus) PGK-1プロモーター, 大腸菌 (Escherichia coli)ネオマイシン耐性遺伝子
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS lett., 462, 335-340 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Dr. Kikuko Watanabe is requested.
DepositorKikuko Watanabe (Kansai Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data